HPLC-DAD-APCI-MS as a Tool for Carotenoid Assessment of Wild and Cultivated Cherry Tomatoes

Carotenoids are naturally occurring fat-soluble pigments found in many organisms. Because of their extensively conjugated carbon–carbon double bond system, carotenoids potent antioxidants. Although the most abundant carotenoid and best singlet oxygen quencher red tomatoes is lycopene, profiles may vary between genotypes. The objective this work was to perform profile indentification using HPLC-DAD-APCI-MS ten wild cherry tomato accessions one cultivated tomato. A mixture hexane/acetone/ethanol (50:25:25) 0.1% BHT used for extraction. For separation, a C30 column at 30 °C with gradient consisting methanol, methyl-tert-butyl ether, water analysis. Ten major were quantified within samples. All present different quantities carotenoids. Wild had more lycopene content that commercial tomato, whereas yellow no lycopene. From functional viewpoint, higher concentrations could play an antioxidant activity measured from IAC401, IAC426, LA1480, IAC391, LA2692. This trait means these germplasms be targets activities. To our knowledge, first time has been analyze both functionally promising suitable projects social implementation local scale.